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Catalog Number | orb640125 |
---|---|
Category | Antibodies |
Description | Clone CS4 is one of four clones to EBV. Each clone reacts with different epitopes on the hydrophilic C-terminus of the cytoplasmic domain of LMP-1. This antibody is specific to 60kDa latent membrane protein (LMP-1) encoded by the BNLF1 gene of the EBV. It stains strongly with EBV-positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis. EBV, also designated human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV infects B cells and, though often asymptomatic, it can cause infectious mononucleosis, a disease characterized by fatigue, fever, sore throat and muscle soreness. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CS4 |
Tested applications | IHC-P, WB |
Reactivity | Virus |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant fusion protein containing the sequence of bacterial beta-galactosidase and the carboxyl half of EBV-encoded LMP was used as the immunogen for the EBV antibody. |
Antibody Type | Primary Antibody |
Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This EBV antibody is available for research use only. |
Storage | Store the EBV antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE Hodgkin's Lymphoma with EBV antibody (clone CS4). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free EBV antibody (clone CS4) as confirmation of integrity and purity.
ELISA, IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |