E2F1 Rabbit mAb [KD Validated] Antibody

SKU: orb1951294

Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution Range: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human E2F1
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of HeLa cells, using E2F1 Antibody.

Immunohistochemical analysis of paraffin-embedded human ovary carcinoma, using E2F1 Antibody.

Western blot analysis of E2F1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.

Quick Database Links

UniProt

Q01094