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Description
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Dilution Range | WB - 1:1000 |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This E2 tag antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide of E2 tag. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: Anti-E2 tag Antibody at 1:1000 dilution (upper) or GAPDH (lower). Lane 1: 293T/17 transfected with 6tag lysate (1ug). Lane 2: Non-transfected 293T/17 lysate (1ug). Lane 3: 6tag recombinant protein lysate (0. 5ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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E2 tag Antibody (orb1925662)
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