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Catalog Number | orb1931727 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This E1 Ubiquitin (UBE1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1026-1058 amino acids from the C-terminal region of human E1 Ubiquitin (UBE1). |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Predicted Reactivity | Rabbit, Rat |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
UniProt ID | P22314 |
MW | 117849 Da |
Tested applications | IHC-P, WB |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:50~100 |
Antibody Type | Primary Antibody |
Clone Number | RB04330 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Ubiquitin-like modifier-activating enzyme 1, Prote Read more... |
Note | For research use only |
NCBI | NP_695012.1, NP_003325.2 |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UBE1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-UBE1 Pab is used in Western blot to detect UBE1 in HL-60 cell lysate.
Western blot analysis of E1 Ubiquitin (UBE1) Antibody (C-term) in mouse stomach tissue lysates (35 ug/lane). UBE1 (arrow) was detected using the purified Pab.