E-selectin, Human, mAb ENA2 Antibody

SKU: orb1108788

Description

Mouse monoclonal antibody to E-selectin

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: Functional Studies, IA, IF, IHC-Fr
• Dilution Range: For immunohistochemistry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
• Reactivity: Human
• Application Notes:
  FS: the antibody can be used for adhesion inhibition studies for which the antibody is most efficient and prevents adhesion of leucocytes via Fc receptors. (Ref.3) IF: In vitro cultured cells can be fixed with 1% paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 µg/ml) in tissue culture medium at 37°C. As negative control it is advised to use a control murine IgG1 antibody.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
• Buffer/Preservatives: 0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only