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Catalog Number | orb1294258 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to E selectin |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / Extracellular domain corresponding to Human E selectin / CD62E |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200 |
Conjugation | Unconjugated |
Target | E-selectin | CD62E |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti E-selectin/CD62E antibody at 1/500 dilution. Lysates at 50 μg per lane, Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294258 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-selectin/CD62E with orb1294258 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-selectin/CD62E with orb1294258 at 1/100.
Anti E-selectin/CD62E antibody at 1/500 dilution. Lysates at 50 μg per lane, Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294258 overnight at 4°C.
FC, ICC, IHC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IHC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
98.00% | |
61.6 kDa (predicted). Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |