E. coli O157 Antibody

• Catalog Number: orb750032
• Category: Antibodies
• Description: This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9.88
• Tested applications: ELISA, IF
• Reactivity: E. coli
• Isotype: Mouse IgM, kappa
• Immunogen: A crude sonicate of E. coli O157 was used as the immunogen for the E. coli O157 antibody.
• Dilution range: ELISA: 1-5ug/ml for coating (order BSA/sodium azide-free format),Immunofluorescence: 1-2ug/ml
• Purity: PEG precipitation followed by dialysis in 1X PBS
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This E coli O157 antibody is available for research use only.
• Storage: Store the E. coli O157 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the E. coli O157 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.