E-Cadherin Antibody / CDH1

SKU: orb606394

Description

Cadherins behave as both receptors and ligands for other molecules. During development, their behavior assists in properly positioning cells: they are responsible for the separation of the different tissue layers, and for cellular migration. In the very early stages of development, E-cadherin (epithelial cadherin) is most greatly expressed. The E-cadherin-catenin complex plays a key role in cellular adhesion; loss of this function has been associated with greater tumour metastasis. [Wiki]

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant E-Cadherin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: CDH1/2208R
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant E-Cadherin antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone CDH1/2208R, green) and Reddot nuclear stain (red).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with recombinant E-Cadherin antibody (clone CDH1/2208R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human MCF-7 cell lysate with recombinant E-Cadherin antibody (clone CDH1/2208R). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).

Flow cytometry testing of human MCF7 cells with recombinant E-Cadherin antibody (clone CDH1/2208R); Red = isotype control, Blue = recombinant E-Cadherin antibody.

SDS-PAGE analysis of purified, BSA-free recombinant E-Cadherin antibody (clone CDH1/2208R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P12830