DYNC1H1 Antibody (C-term)

SKU: orb1926371

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This DYNC1H1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4202-4236 amino acids from the C-terminal region of human DYNC1H1. Antigen Region: 4202-4236 aa.
• Target: DYNC1H1 (HGNC:2961)
• Molecular Weight: 532408 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cytoplasmic dynein 1 heavy chain 1, Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic, DYNC1H1, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325

All lanes: Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution. Lane 1: U-87 MG whole cell lysates. Lane 2: PC-3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 532 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: PC-3 whole cell lysate. Lane 3: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 532 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining DYNC1H1 in human testis sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

DYNC1H1 (HGNC:2961)

UniProt

Q14204