DYKDDDDK Tag Antibody (Equivalent to Sigma's FLAG M2 Antibody): HRP

SKU: orb413742

Description

Rabbit polyclonal antibody against DYKDDDDK Tag conjugated to HRP

Images & Validation

• Tested Applications: WB
• Dilution range: WB (1:1000)
• Reactivity: All
• Application Notes:
  A 1:1000 dilution of SPC-720 was sufficient for detection of DYKDDDDK Tag in 15 µg of DYKDDDDK (FLAG) Positive Control Lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH).
• Target: DYKDDDDK Tag (Equivalent to Sigma's Anti-FLAG M2)
• Purification: Peptide Affinity Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

DDDDK epitope tag, DDDK, ddk, DYKDDDDK, DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG, FLAG tag

Dot Blot analysis using Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody. Tissue: DYKDDDDK (FLAG) Positive Control Lysate. Primary Antibody: Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.

Quick Database Links

Gene Symbol

DYKDDDDK Tag (Equivalent to Sigma's Anti-FLAG M2)