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DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG M2 Antibody): Biotin
Catalog Number: orb413740
| Catalog Number | orb413740 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody against DYKDDDDK Tag conjugated to Biotin |
| Target | DYKDDDDK Tag |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Biotin |
| Reactivity | All |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
| Immunogen | Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH). |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Dilution range | WB (1:1000) |
| Application notes | A 1:1000 dilution of SPC-720 was sufficient for detection of DYKDDDDK Tag in 15 µg of DYKDDDDK (FLAG) Positive Control Lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
| Storage | Conjugated antibodies should be stored according to the product label |
| Alternative names | DDDDK epitope tag antibody, DDDK antibody, ddk ant Read more... |
| Note | For research use only |
Dot Blot analysis using Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody. Tissue: DYKDDDDK (FLAG) Positive Control Lysate. Primary Antibody: Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.
