DYKDDDDK Tag (FLAG) Antibody Agarose Conjugated

SKU: orb344560

Description

Mouse monoclonal antibody against DYKDDDDK (FLAG tag) conjugated to Agarose.

Research Area

Molecular Biology

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: IP: 10 µL resin binds >1 µg FLAG fusion protein
• Application Notes:
  Anti-DYKDDDDK Affinity Gel has been tested by SDS-Page, IP, and western blot and is optimally suited for immunoprecipitation and purification of FLAG tagged fusion proteins. Anti-DYKDDDDK Affinity Gel antibody recognizes the FLAG epitope tag fused to either the amino- or carboxy- terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide for competitive elution to recover fusion protein (see protocol). Refer to the Protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 29E4.G7
• Immunogen: Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
• Purity: Anti-DYKDDDDK Affinity Gel is a purified mouse IgG2a monoclonal antibody coupled to activated agarose. This product is intended for purification of proteins containing the FLAG epitope tag sequence. Binding Specificity: Anti-DYKDDDDK Affinity Gel binds the FLAG epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells. D-Y-K-D-D-D-D-K peptide is recommended for competitive elution to recover fusion protein (see protocol).
• Conjugation: Agarose

Storage & Handling

• Storage: Store vial at 4°C prior to opening.
• Form/Appearance: Suspension of agarose beads
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.99 mg
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

mouse anti-FLAG tag agarose gel, mouse anti-DYKDDDDK agarose gel, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K, DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK antibody