DYKDDDDK Tag (Anti-FLAG) Antibody Peroxidase Conjugated

• Catalog Number: orb344540
• Category: Antibodies
• Description: DYKDDDDK antibody (Peroxidase)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 29E4.G7
• Tested applications: ELISA, IHC, WB
• Isotype: IgG2a
• Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:10,000
• Form/Appearance: Lyophilized
• Purity: This antibody is directed against the FLAG epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: mouse anti-FLAG tag HRP conjugation, Enterokinase
• Note: For research use only
• Application notes: Anti-FLAG Peroxidase has been tested by ELISA and western blot. This antibody is optimally suited for monitoring the expression of FLAG tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.
• Expiration Date: 12 months from date of receipt.

Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1000 for overnight at 4°C. Secondary antibody: none. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: ~60 kDa, ~60 kDa.