Dvl1 Antibody

• Catalog Number: orb1248473
• Category: Antibodies
• Description: Dvl1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-PQLEEAPLTVKSD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80kDa band observed in lysates of cell line NIH3T3 (calculated MW of 75.4kDa according to NP_034221.3). An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 80kDa
• Target: Dvl1
• UniProt ID: P51141
• NCBI: NP_034221.3, NP_001343310.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Dvl1, dishevelled, dsh homolog 1 (Drosophila), RP2
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80kDa band observed in lysates of cell line NIH3T3 (calculated MW of 75.4kDa according to NP_034221.3). An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248473 (1 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248473 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.