DVL1 Antibody (Center)

SKU: orb1787884

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:25, WB: 1:2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This DVL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-470 amino acids from the Central region of human DVL1. Antigen Region: 442-470 aa.
• Target: DVL1
• Molecular Weight: 75187 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Segment polarity protein dishevelled homolog DVL-1, Dishevelled-1, DSH homolog 1, DVL1

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).

All lanes: Anti-DVL1 Antibody (Center) at 1:2000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 2: PC-3 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: MDA-MB453 whole cell lysate. Lane 5: human kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 75 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

DVL1

UniProt

O14640

RefSeq (Protein):NP_004412.2