DUX4 Antibody (FITC)

SKU: orb147958

Description

Mouse monoclonal to DUX4 (FITC). DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain transcription factor 1. Clinically it is a facioscapulohumeral muscular dystrophy candidate gene that appears to have a toxic gain of function (2-4). In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle and possibly other differentiated tissues..

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), ICC/IF (1:1000)
• Reactivity: Human, Mouse
• Application Notes:
  1 µg/ml of SMC-192 was sufficient for detection of DUX4 in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: P2B1
• Immunogen: C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag
• Target: DUX4
• Molecular Weight: 45kDa
• Purification: Protein G Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Double homeobox 4, Double homeobox 10, DUX10, Double homeobox protein 4/10, DUX4L, DUX4L1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DUX4 Monoclonal Antibody, Clone P2B1. Tissue: C2C12 myoblast cells. Species: Mouse. Primary Antibody: Mouse Anti-DUX4 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Cells transfected with pCS2+DUX4.

Western Blot analysis of Mouse C2C12 cell lysate showing detection of DUX4 protein using Mouse Anti-DUX4 Monoclonal Antibody, Clone P2B1. Primary Antibody: Mouse Anti-DUX4 Monoclonal Antibody at 1:1000. Cells transfected with pCS2+DUX4 which, contains an additional upstream start site.

Quick Database Links

Gene Symbol

DUX4

UniProt

F5GZ66

NCBI Gene ID

22947

RefSeq (Protein):NP_149418.3