DUSP5 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499335

Description

DUSP5 Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:200-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: R1A10
• Immunogen: KLH conjugated synthetic peptide derived from human DUSP5
• Target: DUSP5
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Dual specificity protein phosphatase 5; Dual specificity protein phosphatase hVH 3; Dual specificity protein phosphatase hVH3; DUSP 5; DUSP; HVH 3; HVH3; Serine/threonine specific protein phosphatase; VH 3; VH1 like phosphatase 3; VH3; DUS5_HUMAN.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HCT-116, 2: Raji, 3: K562, 4: C2C12, 5: PC12, Protein loading quantity: 20 ug, Exposure time: 60 s, Predicted MW: 42 kDa, Observed MW: 42 kDa.

Tissue: Human bladder carcinoma, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high, pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499335.

Tissue: Human bladder carcinoma, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue).

Tissue: Human papillary thyroid carcinoma, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499335.

Quick Database Links

Gene Symbol

DUSP5

UniProt

Q16690