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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | WB: 1:500-1:2000 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 130-270 of human DUSP12 (NP_009171.1). |
| Molecular Weight | 36kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of various lysates using DUSP12 Rabbit pAb (orb539257) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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Protocol Information
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Jiaxuan He 1 2, Siyuan Li 1, Yunpeng Teng 1, Hongfei Xiong 1, Zhuang Wang 1, Xiaoyao Han 1, Wei Gong 2, Ya Gao Increasing expression of dual-specificity phosphatase 12 mitigates oxygen-glucose deprivation/reoxygenation-induced neuronal apoptosis and inflammation through inactivation of the ASK1-JNK/p38 MAPK pathway Autoimmunity ., 57, 2345919 (2024)
Applications
Reactivity
DUSP12 Rabbit pAb (orb539257)
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