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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | WB: 1:500-1:2000 |
| Reactivity | Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1312-1551 of human DUOX1 (NP_059130.2). |
| Molecular Weight | 180kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DUOX1, LNOX1, NOXEF1, THOX1
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Western blot analysis of extracts of various cell lines, using DUOX1 antibody (orb539256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Quick Database Links
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Carla Raggi 1, Francesca Spadaro 2, Fabrizio Mattei 1, Adriana Rosa Gambardella 1, Francesco Noto 1, Sara Andreone 1, Michele Signore 3, Giovanna Schiavoni 1, Isabella Parolini 1 4, Claudia Afferni Eosinophil-Airway Epithelial Cells crosstalk reveals the eosinophils-mediated DUOX1 up-regulation in a murine allergic inflammation setting J Leukoc Biol, (2024)
Applications
IF
Reactivity
Mouse
Cui, Panpan et al. Vitamin D Attenuates Hypoxia-Induced Injury in Rat Primary Neuron Cells through Downregulation of the Dual Oxidase 1 (DUOX1) Gene Med Sci Monit, 26, e925350 (2020)
DUOX1 Rabbit pAb (orb539256)
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