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Catalog Number | orb1937360 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB31275 |
Tested applications | IF, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 83901 Da |
Target | This DTNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 692-721 amino acids from the C-terminal region of human DTNA. |
UniProt ID | Q9Y4J8 |
NCBI | NP_116763.1, NP_001185873.1, NP_001185874.1, NP_001185867.1, NP_116762.2, NP_001383.2, NP_001185871.1, NP_001185869.1, NP_001381.2, NP_001382.2, NP_116760.2, NP_116761.2, NP_116757.2, NP_001185868.1, NP_001121647.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Dystrobrevin alpha, DTN-A, Alpha-dystrobrevin, Dys Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-DTNA Antibody (C-term) at 1:1000 dilution. Lane 1: U-87MG whole cell lysate. Lane 2: C2C12 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-DTNA Antibody (C-term) at 1:2000 dilution + HepG2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of DTNA Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
DTNA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DTNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.