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Catalog Number | orb1251987 |
---|---|
Category | Antibodies |
Description | dsDNA Antibody [DRNC1-1] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | DRNC1-1 |
Tested applications | ICC, IF, IHC-P |
Reactivity | Human |
Isotype | IgG3, kappa |
Immunogen | Cell nuclei were used as the immunogen for the dsDNA antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Immunofluorescence: 1-2 ug/mlImmunocytochemistry (Acetone-fixed): 0.5-1 ug/ml for 30 min at RTImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Titering of the dsDNA antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
UniProt ID | NotKnown |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Note | For research use only |
Application notes | Immunofluorescence: 1-2 ug/mlImmunocytochemistry (Acetone-fixed): 0.5-1 ug/ml for 30 min at RTImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Titering of the dsDNA antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human tonsil with dsDNA antibody
IHC testing of FFPE human colon carcinoma with dsDNA antibody
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