dsDNA Antibody [AE-2]

SKU: orb1252392

Description

dsDNA Antibody [AE-2]

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-P
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLImmunocytochemistry (Acetone-fixed): 0.5-1 µg/mL for 30 min at RTImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RT (1)Optimal dilution of the dsDNA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3, kappa
• Clone No.: AE-2
• Immunogen: Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

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