DRAM1 Antibody

SKU: orb1239379

Description

DRAM1 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-DRAM antibody (orb1239379) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human DRAM. The immunogen is located within amino acids 170-220 of DRAM.
• Target: DRAM1
• Molecular Weight: Predicted: 14kD, 26kDObserved: 14 kD, 26kD
• Purification: DRAM Antibody is affinity chromatography purified via peptide column.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: DRAM Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

DRAM Antibody: DRAM, DRAM, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: DRAM orb1239379 (0.5 µg/mL), DRAM orb1239371 (2 µg/mL), beta-actin (1 µg/mL) and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of DRAM in Human Liver Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver tissue labeling DRAM with orb1239379 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of DRAM in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-DRAM antibody (orb1239379) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Induced Expression of DRAM by CQ in Mouse Neural Precursor Cells (NPCs) (Walls et al., 2010). WT and p53-deficient NPC cells were treated with or without 25µM CQ for 24hr. CQ treatment caused the increased expression level in both DRAM dimer (32kD) and monomer (16kD) compared to the untreated controls. WB results show DRAM induction was p53-dependent.

Regulated Expression Validation of DRAM in Heterozygous SOD2 KO Mice (Mehta et al., 2011). DRAM expression level detected by anti-DRAM antibodies (orb1239379) decreased in striatum of SOD -/+ KO mice (fig. d) as compared to WT mice.

Quick Database Links

Gene Symbol

DRAM1

UniProt

Q8N682

RefSeq:AAH18435