Dp44mT

SKU: orb1300435

Description

Dp44mT is a potent and selective iron chelator with demonstrated antitumor activity. It has been investigated in vitro and in vivo for its potential in cancer research, particularly for targeting iron-dependent proliferation in various malignancies.

Research Area

Cell Biology, Immunology & Inflammation

Key Properties

• CAS Number: 152095-12-0
• MW: 285.4
• Purity: >98%
• Formula: C₁₄H₁₅N₅S
• SMILES: CN(C)C(=S)NN=C(c1ccccn1)c1ccccn1
• Target: Chelator
• Solubility: Soluble in DMSO (up to at least 25 mg/ml)

Bioactivity

• In Vivo:
  Dp44mT (0.4 mg/kg, i.v.) inhibits M109 tumor implanted in CD2F1 mice by a dose-dependent manner.
• In Vitro:
  In MDA-MB-231 cells, Dp44mT selectively targets towards topoisomerase topo2α resulting in DNA damage. Dp44mT inhibits antiproliferative effects significantly with IC50 of 30 nM, 60 nM, and 60 nM for SK-N-MC, SK-Mel-28, and MCF-7 cells, respectively, while showing no effects on normal MRC-5 fibroblasts. In SK-N-MC neuroepithelioma and M109 cells,Dp44mT inhibits cellular Fe uptake from Fe-Tf , and induces cell apoptosis. Dp44mT, as a Pgp substrate, also overcomes multidrug resistance by the hijacking of lysosomal P-glycoprotein (Pgp).
• Cell Research:
  SK-N-MC, SK-Mel-28, MCF-7 and MRC-5 cells are incubated in the presence and absence of DFO, 311, 3-aminopyridine-2-carboxaldehyde thiosemicarbazone, doxorubicin, and the DpT series of chelators (0-25 μM) for 72 hours at 37°C. The effect of the chelators on proliferation is examined using the MTT assay.
• Animal Research:
  CD2F1 mice that are implanted M109 tumors intravenous inject Dp44mT (dissolved in Propylene glycol) 4 mg/kg in twice daily.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Di-2-pyridylketone-4,4,-dimethyl-3-thiosemicarbazone

Compound Identifiers

PubChem CID

10334137