Doxycycline

SKU: orb1307747

Description

Doxycycline

Research Area

Infectious Disease & Virology, Pharmacology & Drug Discovery, Protein Biochemistry

Key Properties

• CAS Number: 564-25-0
• MW: 444.43
• Purity: 99.88% (May vary between batches)
• Formula: C₂₂H₂₄N₂O₈
• SMILES: [H][C@@]12[C@@H](C)c3cccc(O)c3C(=O)C1=C(O)[C@]1(O)C(=O)C(C(N)=O)=C(O)[C@@H](N(C)C)[C@]1([H])[C@H]2O
• Target: Antibiotic,Antibacterial,Parasite,ribosome,MMP
• Solubility: 10% DMSO+90% Saline:4 mg/mL (9 mM);DMSO:200 mg/mL (450.01 mM)

Bioactivity

• Target IC50:
  A549 cells:1.06 μM|Pancreatic Cancer cells (48 h):987.5 μM|NCI-H446 cells:1.70 μM|Pancreatic Cancer cells (96 h):50.02 μM|Pancreatic Cancer cells (72 h):99.64 μM|chlamydial isolates:0.497 μg/mL
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• In Vivo:
  METHODS: To detect anti-tumor activity in vivo, Doxycycline (50 mg/kg) was administered intraperitoneally once daily for fifteen days to BALB/c mice harboring human mammary carcinoma tumor 4T1. RESULTS: Doxycycline delayed tumor growth and reduced the number of tumor-associated macrophages and blood vessels. METHODS: To assay antimicrobial activity in vivo, Doxycycline (40 mg/kg) was administered intraperitoneally to BALB/c mice exposed to B. pseudomallei 1026b twice daily for two weeks. RESULTS: Doxycycline-treated mice survived 100% of the time, and no B. pseudomallei DNA was amplified from the lungs or spleens of the majority of the surviving mice.
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• In Vitro:
  METHODS: Twenty-two tumor cells were treated with Doxycycline for 48 h. Cell viability was measured by MTT. RESULTS: Lung cancer cells were more sensitive to Doxycycline than most other cell lines. NCI-H446 and A549 cells were sensitive to Doxycycline with IC50s of 1.70 μM and 1.06 μM, respectively. METHODS: Human pancreatic cancer cells PANC-1 were treated with Doxycycline (10-40 μg/mL) for 8-24 h. The cell cycle was detected by Flow Cytometry. RESULTS: Doxycycline caused the cells to arrest in the G1-S phase of the cell cycle.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Parasite, MMP, LNT-229, inhibit, Matrix metalloproteinases, MCF 12A, Inhibitor, G55, Doxytetracycline, Doxiciclina, Doxycycline, Doxycyclinum, antibacterial activity, anti-proliferative activity, Antibiotic, Bacterial, 30S ribosome, 16S ribosome, 293T, SVG, U343, Vibramycin

Compound Identifiers

PubChem CID

54671203