Donkey IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb346730

Description

Donkey IgG (H&L) antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Reactivity: Donkey
• Application Notes:
  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This product has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Donkey IgG whole molecule
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Donkey IgG, and Donkey Serum.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 10.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Rabbit anti-Donkey IgG HRP conjugated Antibody, Rabbit anti-Donkey IgG Peroxidase Conjugated antibody