DOLK Antibody (Center)

SKU: orb1928254

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD45923
• Immunogen: This DOLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-339 amino acids from the Central region of human DOLK. Antigen Region: 312-339 aa.
• Target: DOLK
• Molecular Weight: 59268 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dolichol kinase, Transmembrane protein 15, DOLK, KIAA1094, TMEM15

Western blot analysis of DOLK Antibody (Center) in 293 cell line lysates (35 ug/lane). DOLK (arrow) was detected using the purified Pab.

DOLK Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with DOLK Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

DOLK

UniProt

Q9UPQ8

RefSeq (Protein):NP_055723.1