DNMT3L Antibody

SKU: orb345431

Description

DNMT3L antibody

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution range: ELISA: 1:1000 - 1:3000, IHC: 1:500, IP: 1:100, WB: 1:500
• Reactivity: Human
• Application Notes:
  Anti-DNMT3L antibody has been tested for use in ELISA against the immunizing peptide. This antibody has been tested in immunohistochemistry and western blot. This antibody works for western blotting only following immunoprecipitation. The expected molecular weight of human DNMT3L is 43.6 kDa. Reactivity in other immunoassays is unknown.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.
• Purity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. Reactivity with DNMT3L from other species has not yet been tested.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.11 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-DNMT3L antibody, DNA (cytosine-5)-methyltransferase 3-like, DNMT3L, DNMT 3L, DNMT-3L

Affinity Purified Rabbit anti-DNMT3L was used at a 1:1500 dilution to detect human DNMT3L by western blot after immunoprecipitation using the same antibody. Transfection of U2OS cells (10cm dish) was accomplished using 3 µg of GAL4-DNMT3L. Protein extraction using IPH at 150mM.

Immunohistochemistry Rabbit Anti-Human DNMT3L Antibody. Antibody: Rabbit Anti-Human DNMT3L Antibody at 1:500. Antigen Retrieval: HIER pH6.2 Staining: Nuclear staining of testicular germ cells (Left); Neg Ctrl (Right) Human testis 40X.

The conserved N-terminal cysteine-rich region, the PHD-like motif, characteristic of the Dnmt3 family is shown. The C-terminal domain is the catalytic methyltransferase domain present in Dnmt3a and Dnmt3b whereas the key catalytic motifs are absent in Dnmt3L. Numbers indicate amino acid residues.

Quick Database Links

UniProt

Q9UJW3

RefSeq (Protein):NP_787063.1