DNMT3A Antibody (Center R478)

• Catalog Number: orb1935628
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This DNMT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from the Central region of human DNMT3A.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9Y6K1
• MW: 101858 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB35103
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA
• Note: For research use only
• NCBI: NP_783329.1, NP_715640.2, NP_072046.2, NP_783328.1

DNMT3A Antibody (Center R478) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).

Anti-DNMT3A Antibody (R478WMutant) at 1:1000 dilution + U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 102 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DNMT3A Antibody (Center R478WMutant) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DNMT3A Antibody (Center R478WMutant) for immunohistochemistry. Clinical relevance has not been evaluated.