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DNMT3A Antibody (Center R478)
Description
Images & Validation
−| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 101858 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |

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DNMT3A Antibody (Center R478) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).

Anti-DNMT3A Antibody (R478WMutant) at 1:1000 dilution + U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 102 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DNMT3A Antibody (Center R478WMutant) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DNMT3A Antibody (Center R478WMutant) for immunohistochemistry. Clinical relevance has not been evaluated.
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Protocol Information
DNMT3A Antibody (Center R478) (orb1935628)
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