DNMT3A Antibody (Center R478)

SKU: orb1935628

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD57325
• Immunogen: This DNMT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from the Central region of human DNMT3A. Antigen Region: 457-486 aa.
• Target: DNMT3A
• Molecular Weight: 101858 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A

DNMT3A Antibody (Center R478) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).

Anti-DNMT3A Antibody (R478WMutant) at 1:1000 dilution + U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 102 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DNMT3A Antibody (Center R478WMutant) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DNMT3A Antibody (Center R478WMutant) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

DNMT3A

UniProt

Q9Y6K1

RefSeq (Protein):NP_783328.1, NP_715640.2, NP_783329.1, NP_072046.2