DNAJC5G Antibody

SKU: orb1248531

Description

DNAJC5G Antibody

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 28kDa band in Human Testis lysates after 0.05µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.4kDa according to provisional NP_775921.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining primarily in spermatogonia and in cellular membrane. Recommended concentration, 5-10µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DSGRKYQQNVQSQP
• Target: DNAJC5G
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNAJC5G, DNAJ (Hsp40) homolog, subfamily C, member 5 gamma, CSP-gamma, FLJ40417, DNAJ homolog subfamily C member 5G, cysteine string protein-gamma, dnaJ homolog subfamily C member 5G, gamma cysteine string protein, gamma-CSP, gamma-cysteine string protein

orb1248531 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

DNAJC5G

UniProt

Q8N7S2

RefSeq (Protein):NP_775921.1