DNAJB6 Antibody

• Catalog Number: orb1256633
• Category: Antibodies
• Description: DNAJB6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 40kDa
• Target: DNAJB6
• UniProt ID: O75190
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: DNAJB6, DKFZp566D0824, DnaJ, FLJ42837, HHDJ1, HSJ-
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using DNAJB6 antibody (orb1256633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse ileum using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using DNAJB6 antibody (orb1256633). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.