You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1257617 |
---|---|
Category | Antibodies |
Description | DNAJB14 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DNAJB14 (NP_001026893.1). |
Concentration | batch dependent |
Dilution range | WB: 1:1000 - 1:4000IF: 1:100 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 42kDa |
Target | DNAJB14 |
UniProt ID | Q8TBM8 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | EGNR9427, PRO34683, dnaJ homolog subfamily B membe Read more... |
Note | For research use only |
Application notes | WB: 1:1000 - 1:4000IF: 1:100 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using DNAJB14 antibody (orb1257617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of Hela cells using DNAJB14 antibody (orb1257617) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Filter by Rating