DNAJB14 Antibody

SKU: orb1257617

Description

DNAJB14 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:1000 - 1:4000IF: 1:100 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DNAJB14 (NP_001026893.1).
• Target: DNAJB14
• Molecular Weight: Observed: 42kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EGNR9427, PRO34683, dnaJ homolog subfamily B member 14, DnaJ (Hsp40) homolog, subfamily B, member 14

Western blot analysis of extracts of various cell lines, using DNAJB14 antibody (orb1257617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of Hela cells using DNAJB14 antibody (orb1257617) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

DNAJB14

UniProt

Q8TBM8