DNAJB1 Antibody, KO Validated

• Catalog Number: orb1274448
• Category: Antibodies
• Description: DNAJB1 Antibody, KO Validated
• Target: DNAJB1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).
• UniProt ID: P25685
• MW: Observed: 39kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DnaJ homolog subfamily B member 1, DnaJ protein ho
• Research Area: Signal Transduction
• Note: For research use only

Western blot analysis of extracts of various cell lines, using DNAJB1 antibody (orb1274448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Western blot analysis of extracts from normal (control) and DNAJB1 knockout (KO) 293T cells, using DNAJB1 antibody (orb1274448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of U2OS cells using DNAJB1 antibody (orb1274448). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using DNAJB1 antibody (orb1274448) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using DNAJB1 antibody (orb1274448) at dilution of 1:100. Blue: DAPI for nuclear staining.