DNA Ligase IV Rabbit mAb [KD Validated] Antibody

SKU: orb1950882

Description

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human DNA Ligase IV
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; LIG4; LIG4S; Ligase IV;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of DNA Ligase IV expression in Ramos cell lysate.

Quick Database Links

UniProt

P49917