DNA ligase 3 Antibody

SKU: orb556078

Description

Rabbit polyclonal antibody to DNA ligase III

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

• Tested Applications: ICC, IHC-P, WB
• Dilution Range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Reactivity: Human, Mouse
• Application Notes:
  Positive control: 293T , A431 , HeLa , HepG2

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DNA ligase III. The exact sequence is proprietary.
• Target: DNA ligase III
• Molecular Weight: 113
• Purity: Purified by antigen-affinity chromatography.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Buffer/Preservatives: PBS, 20% Glycerol, 0.025% ProClin 300.
• Concentration: 1.01 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA ligase 3 , LIG2 , LIG3alpha

Immunofluorescence analysis of HeLa cells using DNA ligase III antibody

Immunohistochemical staining of A549 xenograft using DNA ligase III antibody

Western blot analysis of Various whole cell using DNA ligase III antibody

Quick Database Links

Gene Symbol

DNA ligase III

UniProt

P49916