Dmrta1 Polyclonal Antibody

SKU: orb1421192

Description

Rabbit polyclonal antibody to Dmrta1.

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Disclaimer: For research use only

Alternative Names

Doublesex- and mab-3-related transcription factor A1, DMRTA1, DMO

Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Dmrta1 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Dmrta1 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: human prostate tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Dmrta1 Polyclonal Antibody, Unconjugated 1: 500, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

UniProt

Q5VZB9