DMPO Antibody

• Catalog Number: orb67377
• Category: Antibodies
• Description: Mouse Anti-DMPO Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N1664A
• Tested applications: AM, ELISA, ICC, IHC, IP, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25µg)
• Conjugation: Unconjugated
• Target: DMPO
• Storage: -20°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxi
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-189 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DMPO using DMPO antibody

Confocal Immunofluorescencence analysis of mouse macrophage cell lines using DMPO antibody