DLC1 Antibody

• Catalog Number: orb1247600
• Category: Antibodies
• Description: DLC1 Antibody
• Target: DLC1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: SVAIRKRSWEEHC
• UniProt ID: Q96QB1
• Tested applications: ELISA, IF, IHC
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Immunohistochemistry:Paraffin embedded Mouse Adrenal Gland. Recommended concentration: 4ug/ml.Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U251 and U2OS cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DLC1, HP, ARHGAP7, STARD12, FLJ21120, p122-RhoGAP,
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_079043.3, NP_872584.2

orb1247600 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and vesicle staining.

orb1247600 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.

orb1247600 (4 ug/ml) staining of paraffin embedded Mouse Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247600 Negative Control showing staining of paraffin embedded Mouse Adrenal Gland, with no primary antibody.