DLC1 Antibody

SKU: orb1247600

Description

DLC1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Immunohistochemistry:Paraffin embedded Mouse Adrenal Gland. Recommended concentration: 4µg/mL.Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U251 and U2OS cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: SVAIRKRSWEEHC
• Target: DLC1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DLC1, HP, ARHGAP7, STARD12, FLJ21120, p122-RhoGAP, deleted in liver cancer 1, Rho-GTPase-activating protein 7, START domain containing protein 12, StAR-related lipid, transfer protein 12, StAR-related lipid transfer (START) domain containing 12, deleted in liver cancer 1 variant 2

orb1247600 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and vesicle staining.

orb1247600 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.

orb1247600 (4 ug/ml) staining of paraffin embedded Mouse Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247600 Negative Control showing staining of paraffin embedded Mouse Adrenal Gland, with no primary antibody.

Quick Database Links

Gene Symbol

DLC1

UniProt

Q96QB1

RefSeq (Protein):NP_872584.2, NP_079043.3