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Item 1 of 4
DLAT Antibody (C-term)
Catalog Number: orb1930555
| Catalog Number | orb1930555 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB26843 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 68997 Da |
| Target | This DLAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 579-607 amino acids from the C-terminal region of human DLAT. |
| UniProt ID | P10515 |
| NCBI | NP_001922.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Dihydrolipoyllysine-residue acetyltransferase comp Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
DLAT Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
DLAT Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DLAT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
DLAT Antibody (C-term) western blot analysis in K562 (lane 1), HepG2 (lane 2), Jurkat (lane 3) cell line lysates (35 ug/lane). This demonstrates the DLAT antibody detected the DLAT protein (arrow).
All lanes: Anti-DLAT Antibody (C-term) at 1:1000 dilution. Lane 1: LNCaP whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
