Disulfiram

SKU: orb1310987

Description

Disulfiram is a specific inhibitor of human acetaldehyde dehydrogenases (ALDH1 & ALDH2) and the pyroptotic executor GSDMD. This dual activity supports its research use in studying alcohol metabolism, cellular pyroptosis, and related inflammatory pathways in both cellular and animal models.

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 97-77-8
• MW: 296.54
• Purity: 95.00% (May vary between batches)
• Formula: C₁₀H₂₀N₂S₄
• SMILES: CCN(CC)C(=S)SSC(=S)N(CC)CC
• Target: Pyroptosis,Apoptosis,Cuproptosis,Dehydrogenase,Interleukin
• Solubility: Ethanol:29.7 mg/mL (100.16 mM);20% Cremophor EL+80% Saline:10 mg/mL (33.72 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.97 mg/mL (10.02 mM);DMSO:250 mg/mL (843.06 mM)

Bioactivity

• Target IC50:
  Topo I:42 ± 8 μM|IMP-7:16.25 ± 1.6 μM|NDM1:1.5 ± 0.6 μM|Topo II:30 ± 9 μM
• In Vivo:
  Disulfiram, clinically employed as an anti-alcoholism agent, potently inhibits both constitutive and 5-FU-induced NF-κB activity in a dose-dependent manner. In the DLD-1 and RKO (WT) cell lines, Disulfiram significantly augments the apoptotic effects of 5-FU and synergistically enhances its cytotoxicity. In melanoma cells, co-treatment with copper or zinc ions reduces cyclin A expression compared to Disulfiram alone and inhibits proliferation in vitro. Moreover, Disulfiram decreases viable cell count, with copper chloride addition markedly intensifying DSF-induced cell death.
• In Vitro:
  Disulfiram inhibits the P-glycoprotein efflux pump, suppresses the transcription factor NF-κB (nuclear factor-κB), reduces angiogenesis, and inhibits tumor growth in mice. Its antitumor activity is associated with in vivo protease inhibition. Disulfiram also induces apoptosis. In severe combined immunodeficient mice with melanoma xenografts, it impedes growth and angiogenesis, an effect that is enhanced by zinc supplementation.
• Cell Research:
  The effect of disulfiram (0.15-5.0 μM) or sodium diethyldithiocarbamate (1.0 μM) on proliferation of malignant cell lines is studied in cultures stimulated with 10% FBS. Cell numbers are quantitated 24 to 72 hours later, as outlined below. In some experiments, disulfiram is added immediately after cells are plated. In other experiments, cells are plated and allowed to grow for 24 to 72 hours before fresh medium with disulfiram is added and cell numbers are assayed 24 to 72 hours later. Synergy is studied between disulfiram and N,N′-bis(2-chloroethyl-N-nitrosourea (carmustine, 1.0-1,000 μM) or cisplatin (0.1-100 μg/mL) added to medium. The effect of metal ions on disulfiram is studied with 0.2 to 10 μM Cu2+ (provided as CuSO4), Zn2+ (as ZnCl2), Ag+ (as silver lactate), or Au3+ (as HAuCl4·3Water) ions added to growth medium, buffered to physiologic pH. To provide a biologically relevant source of copper, medium is supplemented with human ceruloplasmin at doses replicating low and high normal adult serum concentrations (250 and 500 mg/mL).

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin, Interleukin Related, NSC 190940, Inhibitor, inhibit, NSC190940, NSC-190940, Disulfiram, DBH, ALDH1, ALDH, Aldehyde Dehydrogenase (ALDH), Tetraethylthiuram, Tetraethylthiuram Disulfide, Tetraethylthiuram disulfide, TETD, Pyroptosis

Compound Identifiers

PubChem CID

3117