EWP 815

SKU: orb1688824

Description

EWP 815 is a disulfiram analog that potently inhibits Ins(1,4)P2 phosphatase, Ins(1,4,5)P3 5-phosphatase, and dopamine β-hydroxylase. This inhibitor is used in research to study phosphoinositide signaling pathways and catecholamine biosynthesis in both cellular and animal models.

Research Area

Metabolism Research

Key Properties

• CAS Number: 20231-01-0
• MW: 350.59
• Formula: C₁₂H₂₂N₄S₄
• SMILES: C(SSC(=S)N1CCN(C)CC1)(=S)N2CCN(C)CC2
• Target: Phosphatase|||Hydroxylase
• Solubility: DMSO:1 mg/mL (2.85 mM)

Bioactivity

• In Vivo:
  EWP 815 (50 mg/kg; i.p.; administered 30 minutes before euthanasia) inhibits dopamine β-hydroxylase activity in mice, reducing the amount of 3 H-α-Me-NA by 12%.
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• In Vitro:
  EWP 815 (6, 8 μM; 10 min) inhibits dephosphorylation of [3H]Ins(1,4,5)P3 5-phosphatase in particulate and soluble fractions with IC50s of 6 μM and 8 μM, respectively. EWP 815 (30 μM; 1 h) exerts higher inhibition on Ins(1,4)P2 phosphatase rather than Ins(1,4,5)P3 5-phosphatase with the mean [3H]Ins(1,4)P2/mean [3H]InsP recovery ratio of 2.6. EWP 815 (3-300 μM; 30 min) suppresses thyrotropin-releasing hormone (TRH)-stimulated (100 nM) inositol phospholipid production by 30% (100 μM) and 1.8% (300 μM), respectively, without affecting Ins(1,4,5)P3 binding.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dopamine β-hydroxylase, Phosphatase

Compound Identifiers

PubChem CID

30054