Digitonin

SKU: orb1305114

Description

Digitonin is a glycoside detergent widely used in research to permeabilize cell membranes via cholesterol binding. Its membrane-disrupting properties facilitate studies in cell biology and biochemistry, both in vitro and in vivo, and it has been investigated for its antitumor potential.

Key Properties

• CAS Number: 11024-24-1
• MW: 1229.31
• Purity: 98.06%
• Formula: C₅₆H₉₂O₂₉
• SMILES: [H][C@@]12O[C@]3(CC[C@@H](C)CO3)[C@@H](C)[C@]1([H])[C@@]1(C)CC[C@@]3([H])[C@@]([H])(CC[C@@]4([H])C[C@@H](O[C@]5([H])O[C@H](CO)[C@H](O[C@]6([H])O[C@H](CO)[C@@H](O)[C@H](O[C@]7([H])OC[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O[C@]6([H])O[C@H](CO)[C@H](O)[C@H](O[C@]7([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)C[C@]34C)[C@]1([H])[C@@H]2O
• Target: Others
• Solubility: H2O:99.57 mg/mL (81 mM);DMSO:242.5 mg/mL (197.27 mM)

Bioactivity

• Target IC50:
  LLC cells:17.2 µM|CT-26 cells:5 µM|4T1 cells:7.8 µM|RBC membranes (sheep):15.1 µM|MC38 cells:4.3 µM|Caco-2 cells:15.72 ± 0.16 µM|MCF-7 cells:5.23 ± 0.61 µM
• In Vivo:
  METHODS: To determine whether Digitonin enhances the efficacy of CBDCA in vivo, Digitonin (0.2 mL 400 μM) was hepatic arterially infused into LH rats bearing hepatocellular carcinoma tumors, followed by injection of CBDCA 10 min later. RESULTS: Digitonin pretreatment increased the tumor uptake of CBDCA and enhanced the cytotoxic effect of CBDCA.
• In Vitro:
  METHODS: Rat adrenal pheochromocytoma cells PC-12 were treated with Digitonin (10 μg/mL) for 10 min to increase cell permeability. RESULTS: After permeabilization with Digitonin, PC12 cells could be stimulated to release norepinephrine by micromolar concentrations of free Ca2+. METHODS: Human glioblastoma cells U87 were treated with Digitonin (1-5 μg/mL) for 20 min, and cell viability was measured by MTT after overnight cell incubation. RESULTS: 1 μg/mL Digitonin treatment resulted in a slight increase in cell viability, 3 μg/mL Digitonin reduced the growth-promoting effect, and the cell viability was similar to that of the control, and 5 μg/mL Digitonin inhibited the cell viability, which indicated a significant toxicity to the cells. METHODS: Immortalized human T-lymphocytes Jurkat were treated with Digitonin (1-4 μg/mL) for 20 min and cell viability was measured by Resazurin assay. RESULTS: Digitonin treatment resulted in a significant decrease in metabolic activity and this inhibition was concentration-dependent.

Storage & Handling

• Storage: keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cell, cholesterol, detergent, Digitonin, Inhibitor, inhibit, molecules, mild, permeability

Compound Identifiers

PubChem CID

6474107