DHFR Antibody

• Catalog Number: orb626366
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DHFR
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: dihydrofolate reductase
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: DHFR
• UniProt ID: P00374
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of HeLa cells using orb626366 (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-DHFR (IP: orb626366, 3 ug; Detection: orb626366 1:500) with Raji cells lysate 2000 ug.

K-562 cells were subjected to SDS PAGE followed by western blot with orb626366 (DHFR antibody) at dilution of 1:500.

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using orb626366 (DHFR Antibody) at dilution of 1:50.