DHDH Polyclonal Antibody

• Catalog Number: orb1420121
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DHDH.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat
• Conjugation: Unconjugated
• UniProt ID: Q9UQ10
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Protein: liver (mouse) lysate at 30ug; Primary: rabbit Anti-DHDH at 1: 300; Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000; Predicted band size: 36 kD. Observed band size: 36 kD

Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DHDH) Polyclonal Antibody, Unconjugated at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.