DHCR7 Antibody (C-term)

• Catalog Number: orb1938186
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This DHCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-463 amino acids from the C-terminal region of human DHCR7.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9UBM7
• MW: 54489 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:25, IF: 1:10~50, WB: 1:2000, WB: 1:1000, IHC-P: 1:50~100, WB: 1:2000
• Antibody Type: Primary Antibody
• Clone Number: RB17942
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 7-dehydrocholesterol reductase, 7-DHC reductase, P
• Note: For research use only
• NCBI: NP_001157289.1, NP_001351.2

DHCR7 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35 ug/lane). This demonstrates the DHCR7 antibody detected the DHCR7 protein (arrow).

All lanes: Anti-DHCR7 Antibody (C-term) at 1:2000 dilution. Lane 1: Caco2 whole cell lysate. Lane 2: mouse testis lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.Observed band size: 55kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-DHCR7 Antibody (C-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Li-7 whole cell lysate. Lane 3: U-87MG whole cell lysate. Lane 4: mouse testis lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of DHCR7 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

DHCR7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DHCR7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).