DGCR8 Rabbit mAb [KD Validated]

• Catalog Number: orb1951653
• Category: Antibodies
• Description: Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human DGCR8
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:500~1:2000 ICC/IF 1:100~1:500 IP 1:50
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: Q8WYQ5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DGCR8; DGCRK6; Gy1 ; C22orf12; D16Wis2; pasha;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of DGCR8 expression in (1) HEK293 cell lysate; (2) HeLa cell lysate.