Desmoglein 3 Antibody / DSG3

SKU: orb750036

Description

Desmoglein 3 is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. [UniProt]

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting amounts. Titration of the Desmoglein 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: DSG3/1535
• Immunogen: A protein fragment corresponding to the extracellular portion of human DSG3 was used as the immunogen for the Desmoglein 3 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human skin with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE human lung squamos cell carcinoma with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

Quick Database Links

UniProt

P32926