Desmoglein 1 antibody

SKU: orb2276296

Description

Desmoglein 1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:75-1:150
• Reactivity: Human
• Application Notes:
  Positive Control: Tonsil: A moderate to strong desmoglein 1 immunostaining should be seen in a fraction of squamous epithelial cells. Negative Control: Desmoglein 1 immunostaining should be absent in all non-squamous epithelial cell types. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: MSVA-544M
• Immunogen: Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cadherin family member 4; CDHF4; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DSG1; EPKHE; EPKHIA; Pemphigus foliaceus antigen; PPKS1; SPPK1

Quick Database Links

UniProt

Q02413