Desmoglein 1 antibody (MSVA-544M)

• Catalog Number: orb2276296
• Category: Antibodies
• Description: Desmoglein 1 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-544M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2a, kappa
• Immunogen: Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis.
• Dilution range: 1:75-1:150
• Conjugation: Unconjugated
• UniProt ID: Q02413
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Cadherin family member 4; CDHF4; Desmoglein-1; Des
• Note: For research use only
• Application notes: Positive Control: Tonsil: A moderate to strong desmoglein 1 immunostaining should be seen in a fraction of squamous epithelial cells. Negative Control: Desmoglein 1 immunostaining should be absent in all non-squamous epithelial cell types. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Squamous cell carcinoma of the oral cavity with strong Dsg1 positivity of most tumor cells.

Squamous cell carcinoma with variable Dsg1 staining of tumor cells showing only weak staining at the periphery of tumor cell nests.

Squamous epithelium of the skin showing a strong membranous desmoglein 1 immunostaining.