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Catalog Number | orb606942 |
---|---|
Category | Antibodies |
Description | Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | DES/2960R |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the recombinant Desmin antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant Desmin antibody is available for research use only. |
UniProt ID | P17661 |
Storage | Store the recombinant Desmin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant Desmin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
FACS, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Gallus, Hamster, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Gallus, Hamster, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Gallus, Hamster, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Gallus, Hamster, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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