DEPDC1 Antibody (N-term)

• Catalog Number: orb1930508
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB26839
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 92960 Da
• Target: This DEPDC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-38 amino acids from the N-terminal region of human DEPDC1.
• UniProt ID: Q5TB30
• NCBI: NP_001107592.1, NP_060249.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: DEP domain-containing protein 1A, DEPDC1, DEPDC1A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

DEPDC1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEPDC1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

DEPDC1 Antibody (N-term) western blot analysis in U251 cell line lysates (35 ug/lane). This demonstrates the DEPDC1 antibody detected the DEPDC1 protein (arrow).

All lanes: Anti-DEPDC1 Antibody (N-term) at 1:1000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: A431 whole cell lysate. Lysates/proteins at 30 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 93 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.